Journal of Nanomedicine & Nanotechnology

Abstract

Biosynthesis of Gold Nanoparticles by Human Microbiota from Healthy Skins

Pooja Deshpande, Sharvari Gaidhani, Hitendra M, Yogesh Shouche, Narhe R, Richa Singh, Sweety Wadhwani, Utkarsha Shedbalkar, Dhanjay Gond and Balu Ananda Chopade

High moisture content of human allows prevalence of microbial flora as commensals. The present study aimed at isolation, purification, identification of human micro flora found in anticubital fosse in Indian adult individuals and their ability to produce metal nanoparticles. Swabs were collected from 80 healthy individuals consisting of 50% of male. Preliminary morphological identification of nine isolates (HACF 1, 4 -9, 11) was performed for the colonies growing on specific isolation medium. Isolates were identified by API 32 GN system and 16S r RNA gene sequencing. Providencia spp. HACF 11 exhibited the ability to produce cell bound gold nanoparticles by producing a 550nm in UVVisible spectroscopy. It was further confirmed by TEM and SEM analysis. Temperature was found to exhibit profound effect on synthesis with increasing monodispersity producing spherical nanoparticles. With increase in temperature, the time required for synthesis of gold nanoparticles reduced to 24 h.