Journal of Nanomedicine & Nanotechnology

Abstract

Biosynthesis and Characterization of Silver Nanoparticles (Snps) by using Leaf Extracts of Ocimum Sanctum L (Tulsi) and Study of its Antibacterial Activities

Birendra K Bindhani and Ashok K Panigrahi

The present study aimed to synthesis of SNPs (Silver Nanoparticles) or (Green-Silver) in aqueous medium using leaf extracts of Ocimum sanctum L. The synthesized silver nanoparticles (SNPs) were characterized by the different techniques such as UV-vis Spectrophotometer, XRD, FTIR and TEM. The synthesis of silver nanoparticles were confirmed due to the SPR optical absorption band peak at ~440 nm by UV-vis spectrophotometer, nearly 15-45 nm in diameter with spherical in shape by TEM, high crystalline with the Bragg peaks of (111), (200), (220) and (311) plane as the predominant orientation by XRD and FTIR confirmed the presence of methoxy and allyl groups in SNPs. The reduction of Ag metal ions was performed due to the presence of various types of anti-oxidants in the leaves of Ocimum sanctum L. and each having a unique structure and functions. The formations of silver nanoparticles through aqueous medium were fairly stable and which can be incubated up to 4 months in refrigerator. At last, the antimicrobial activities of AgNPs were evaluated using different bacteria strain. It was found that, this biosynthesizing of silver nanoparticles may inhibit the all types of bacterial pathogenic organisms competently. This recent research study opens an innovative design to progress our understanding of how SNPs behave can be optimized to improve human antimicrobial activities.