Journal of Coastal Zone Management

Abstract

Biomedical Potentials of Intertidal Marine Organisms from Singapore

Alfred Wai Ping Seng, Yan Zhi Ong, Wan Yen Lee, Peter Peng Foo Lee, Swee Cheng Lim, Koh Siang Tan and Lik Tong Tan

As part of a pilot scale project on drug discovery from local marine organisms, 19 intertidal marine
organisms from Singapore waters were collected and screened for the presence of biologically-active
natural products. These marine organisms were collected due to the ease of procurement and their relative
abundance. The organic extracts of these organisms were prepared and screened in the brine shrimp
lethality (BSL), the cytotoxicity (MCF-7 and MOLT-4 cell lines), and the quorum sensing inhibition (QSI)
assays. Over 60% of the extracts gave significant biological activities in the BSL and the cytotoxicity
(MOLT-4) assays when tested at 1000 ppm. Three sponge extracts showed moderate antibacterial activity
while a fraction obtained from the gross fractionation of the extract of the marine cyanobacterium, Lyngbya
majuscula (PH2), exhibited anti quorum sensing activity in the QSI assay. Lyngbya majuscula (PH2) also
exhibited exceptional biological properties in the toxicity assays and its extract underwent further
fractionation. The 1H-NMR spectra of the bioactive chromatographic fractions derived from the microalgal
extract indicated the presence of unique lipopeptides. Data from this study provided rationale to initiate
marine natural products research for drug discovery in Singapore.