Global Journal of Biology, Agriculture & Health Sciences

Abstract

Benzene Biodegradation by Bacillus Subtilis in Water and Validation System with Kefir Grains as Biological Indicator

Neves, C.R.S.S & Penna, T.C.V

Benzene is an aromatic molecule, which belongs to a particular class of compounds known as BTEX: benzene, toluene, ethylbenzene and xylene, such compounds harm to the environment and humans. An effective process for the degradation of these compounds is bioremediation. This technique aims the biological oxidation by microorganisms decomposing organic matter in simple substances such as CO2 and H2O. In this work, we studied the kinetics of wellZeno degradation by Bacillus subtilis ATCC 6051. The experiment of biodegradation, early containing 100mg /L of benzene, developed in cell concentration of 1g /L. The saline decontamination level was evaluated with kefir grains, as a biological indicator. The biodegradation of 48% of benzene was confirmed (time less than 2 hours) for cell development. The decontamination of water promoted the development of kefir grains, about 2-3 times greater than the initial concentration, which used as a biological indicator of the biodegradation developed system.