Journal of Aquaculture Research & Development

Abstract

Antibiofilm Competency of Portunus pelagicus Haemolymph and Identification of its Bioactive Compounds

Mahalingam Anjugam, Arokiadhas Iswarya, Thiruselvam Indumathi, Baskaralingam Vaseeharan, Raman Pachaiappan, Narayanan Gopi and Palaniyandi Velusamy

The marine atmosphere may be investigated as a rich source for novel drugs. In past decades, a number of marine-derived compounds have been isolated and identified. The current effort was made on haemolymph of blue swimmer crab Portunus pelagicus to mark its antimicrobial activity against eight pathogenic bacteria of Gram positive, Gram negative and fungus Candida albicans. Agar well diffusion method was put into practice in deliverance of antibacterial and antifungal activity by measuring zone of inhibition. The findings displays, P. pelagicus haemolymph at the concentration of 150 μl can effectively act against all of challenged bacteria and fungus. Besides, the antibiofilm property of haemolymph against selected bacteria and fungus C. albicans which revealed the potential bactericidal and fungicidal effect at 150 μl concentration of haemolymph. Moreover, the antimicrobial and antibiofilm potent of crab haemolymph was confirmed by growth curve analysis, biofilm growth inhibition and protein leakage assay which collectively conclude the ability of haemolymph in microbial growth inhibition. For the first time, the bioactive compounds were screened from the haemolymph of P. pelagicus through Gas chromatography- Mass spectrometry analysis discloses the existence of 15 types of compounds in haemolymph being in charge for antimicrobial activity.