Journal of Clinical & Experimental Pharmacology

Abstract

An In vitro Approach to Evaluate the Anti Adipogenesis Activity of Passiflora edulis f Flavicarpa Degener Leaf Extracts in 3T3-L1 Cell Lines

Saji JA and Veena R

Context: Obesity has been proved to be a complex multifactorial chronic disease in today’s era accelerating the risk for Type 2 diabetes, coronary heart disease, and hypertension. The use of natural products in the management of obesity as well as reducing weight is being carried out by many researchers in the field of pharmacology.
Aim: The present study was carried out to determine the anti-adipogenic as well as anti-hyperlipidemic activities Extracts of Passiflora edulis f. Flavicarpa Degener leaves (EEPE) using 3T3-L1 Murine adipocyte cell lines.
Settings and Design: MTT assay was performed for cell viability as well as inhibition of the extract produced on the differentiation of pre-adipocytes; to study the anti-hyperlipidemic activity we used Oil red O staining method and pancreatic lipase inhibition activity.
Methods and Material: MTT assay was performed for cell viability studies on 3T3-L1 cell lines using the extract. The anti-hyperlipidemic activity of the EEPE in 3T3-L1 cell lines was evaluated by Inhibition of pancreatic lipase and lipid accumulation study using Oil Red O Staining wherein Orlistat and Simvastatin were used as the reference standard respectively.
Statistical analysis used: All the experiments were performed in triplicates and the results were expressed Mean ± SEM where n=3. A student’s t-test was done and a p-value <0.05 was considered to be significant.
Results: In the course of cell viability determination, EEPE exhibited 42.06% at 150 µg concentration which was higher when compared to Simvastatin whose cell viability was 41.36% at 50 µg. The Lipid accumulation was substantially inhibited by test extract and simvastatin at 45.19% and 31.45% respectively whereas the % inhibition of the pancreatic lipase in matured 3T3-L1 adipocytes by the inhibition of breakdown of triglycerides was found to be 60.44% and 49.43% at 450 µg for EEPE and Orlistat respectively.
Conclusion: Thus, we conclude that EEPE has significant hypolipidemic effects on 3T3-L1 cell line, therefore, holds promise as an anti-hyperlipidemic herb.