Pharmaceutica Analytica Acta

Abstract

Ameliorative Role of Dietary Supplemented Conjugated Linolenic Acid against Nicotine-Induced Toxicity in Rats

Krishna Chattopadhyay, Moumita Maity, Satyam Banerjee and Brajadulal Chattopadhyay

Increasing consumption of tobacco in different forms harms almost every organs of our body. Nicotine is the culprit for various physiological repercussions arouse due to the uptake of tobacco. As nutritional status alters the actions, potencies and detoxification of toxicants, the present study was undertaken to evaluate the natural antioxidant efficacy of conjugated linolenic acid present in Karalla seed against nicotine-induced toxicity. Experiments were conducted on male albino rats (120–130 g body weight) by injecting nicotine tartarate (3.5 mg/kg body wt. /day for 15 days) subcutaneously and thereby simultaneously supplementing conjugated linolenic acid (0.5 and 1.0%) to their diets. Nicotine significantly altered serum and liver lipid profiles, lipid peroxidation and activities of antioxidant enzymes. It caused significant decrease of DNA contents (P<0.01) and DNA damage (P<0.001) of liver tissue. Conjugated linolenic acid has the ability to bind with DNA and protein similar to nicotine and thereby ameliorates nicotine-induced toxicity in rats. Thus intake of Karalla that contains conjugated linolenic in its seeds, in our daily diet can effectively attenuate nicotine-induced cellular and genetic damages.