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Abstract
A Versatile Metagenome Purification Method to Identify Uncultivable Bacteria by Denaturing Gradient Gel Electrophoresis (DGGE) from Sediments and Soils
Lourdes Reyes-Escogido, Ingrid Rodríguez-Buenfil, Jesús Valdés, Luis Kameyama, Odila Saucedo Cárdenas and Francisco Martínez- Pérez
Here, we report a versatile metagenome purification method to identify uncultivable species from sediments and soils by nested-PCR-DGGE according to 16S rDNA. This combination of methods uses enzymatic lysis in situ, polyvinylpyrrolidone (PVP), Chelex 100, glass bead-silica gel and chaotropic salts, with the advantage that it can be applied to different soils.